The life cycle of the research project
The purpose of the course is to provide knowledge about the main stages of the idea implementation, planning and execution of the experimental part of a scientific project (preparation phase, selection and setting of methods, design and planning of individual experiments, expansion of project to study the molecular mechanisms underlying the obtained biological effects). The practical part will include the preparation of a project according to one's own idea, but with invented results that confirm the idea. The basic skills and abilities necessary for successful research include the use of laboratory equipment, experimental design, preparation of the solutions, selection of the conditions for determination of enzyme activities, filling the lab notebook as well as preparation of scientific reports and a manuscript. All these issues will be addressed during the course. Practical classes will include video demonstrations of the laboratory work and experiments.
Having a new idea. How to evaluate the quality of the idea? Personal and team projects. Interaction within team projects.
Selection of the minimal and optimal sets of experiments for hypothesis (idea) validation. Determination of the conditions for conducting experiments and selecting the methods. Evaluation of opportunities for the implementation of the idea.
Extension of the project to study the underlying molecular mechanisms.
Statistical processing of the results, generation of tables and graphs.
Documentation of experimental results. Description of the experimental conditions, materials and methods. Preparation of intermediate and final reports. Evaluation of data quality and its value for scientific society.
Reagents from the supplier's catalog/website and bottles
Preparation of solutions
- Calculate the amount of reagents to prepare the solutions
Rules for working with equipment
Filling in the labbook
Working in a team
- Fill in the labbook for the day of work
Types of graphs and their use
- Preparing graphs for different experiments (different groups, time dynamics, concentrations, two-factor)
Determination of measurement conditions and medium composition
Calculation of stock solutions
- Calculate stock solutions for the preparation of the activity determination mixture
- Prepare media of different volumes
Selection of supernatant volumes
Calculation of activity
- Determination of the linear range
- Calculation of enzyme activity based on changes in optical density (per μL of supernatant or protein)