Instrumental methods

General description

This course is focused on instrumental methods of biophysics and molecular biology. Its main goal is to explain what problems can be solved using each of the methods and to teach how to select the simplest and the most effective approach to answer particular experimental question. Practical works will include planning of simple fluorescence or CD experiments  and processing real experimental data from such experiments.

Download of lectures and other teaching material

You need to be registered to the course and to login to the site to see the complete list lectures and teaching material available to download. 
Please consider the copyright. You are allowed to share only teaching material visible to users that is accessible without authorization. 

Details of the curriculum

Course: 4 lectures, 4 practical works

Lectures/Seminars: Volodymyr Shvadchak

Fluorescence-based methods in biology

Fluorescence principles. Fluorophores. Brightness. Solvatochromism. Protein labeling with organic dyes. Tryptophan fluorescence and other natural fluorophores. FRET and its application to study protein interactions. Fluorescence anisotropy.

Methods to determine size of the molecules and low-resolution structural methods

Electrophoresis of proteins and oligonucleotides. DLS. FCS. CD spectroscopy to determine protein structure. IR spectroscopy.

High-resolution structural methods

NMR. Spin. 13C and 15N protein labeling. NMR for protein structure analysis. Solid state NMR. ESR and free radicals. X-ray. Protein crystallization.

Chromatography and mass-spectrometry

 HPLC principle, preparative and analytical applications. Types of columns. Ion exchange chromatography. Size-exclusion. Mass-spectrometry. LC-MS. ESI, MALDI and other ionization methods. Types of mass detectors. Fragmentation. LC-MS in proteomics.

1. Fluorescence

Fluorescence quantum yield. Solvatochromic dyes.
Characterization of protein-membrane interaction using tryptophan fluorescence.

Solving of simple problems on solution concentrations

2. CD spectroscopy

Practice with real data: characterization of protein-membrane interaction using CD spectroscopy.

Problems on concentration determination using UV-absorbance of proteins and on protein labeling.

3. Basics of HTS

Practice with real data: determination of IC50 of inhibitor based on kinetic experiment data

4. Electrophoresis, chromatography and MS

Processing of SDS-PAGE data to quantify fractions proteins in the mixture.

Selection of proper gradient for chromatography experiment.

Fluorescence textbooks
Підручники загального плану
Додаткові матеріали

Advances in Protein Molecular and Structural Biology Methods (2022) (Розділи 7, 8, 10, 12, 14, 16, 20, 21)

Робота з даними

 Пояснення про відображення похибок на графіках. Geoff Cumming, Fiona Fidler, David L. Vaux, Error bars in experimental biology. JCB. (2007)

Undergraduate students
4 Lectures
Practical classes
4 Lesson
2 Months

Employee of the Precarpathian University (Ivano-Frankivsk) and the Institute of Organic and Bioorganic Chemistry (Prague). Doctorate degree in Life sciences (PhD) was obtained from Strasbourg University in 2009 for research work on the development of solvatochromic fluorescent labels for studies of protein interactions.

Предмети курсу

Course: 15 lectures, 6 seminars

Lectures: Halyna Semchyshyn, Galyna Ushakova, Oleh Lushchak

Seminars: Galyna Ushakova, Oleksandra Abrat

Course: 7 lectures, 4 seminars

Lectures: Volodymyr Shvadchak

Seminars: Volodymyr Shvadchak, Ivan Muzalov


Course: 9 lectures, 4 seminars

Lectures: Volodymyr Lushchak, Andriy Luzhetskyy

Seminars: Mariia Bailiak, Volodymyr Lushchak

Course: 13 lectures, 5 seminars

Lectures: Olga Garaschuk, Volodymyr Manko

Seminars: Volodymyr Manko, Volodymyr Merlavsky

Course: 4 lectures

Lectures: Volodymyr Shvadchak, Yury Kovalchuk, Nina Bondarenko