Introduction to Microscopy

General description

This course overviews main modern microscopy techniques used in cell biology. It deals both the theoretical background of the different types of microscopy and with practical aspects of sample preparation, digital data processing and experiment design will be covered. The main objectives of the course are to provide the students with the knowledge necessary to start a practical training on modern fluorescence microscopes and to describe the problems that can be solved by different specific types of microscopy.

Download of lectures and other teaching material

You need to be registered to the course and to login to the site to see the complete list lectures and teaching material available to download. 
Please consider the copyright. You are allowed to share only teaching material visible to users that is accessible without authorization. 

Details of the curriculum

Course: 4 lectures

Lectures: Volodymyr Shvadchak, Yury Kovalchuk, Nina Bondarenko

1. Transmission and fluorescence microscopy

Transmission microscopy, phase contrast. Fluorescence microscopy. Principal schemes of microscopes. Lasers. Filters. Dichroic mirrors. Channels. Digital image collection. Image resolution, micrones and pixels. Confocal microscopy. Z-slices. TIRF. ImageJ (Fiji) software for image processing. Colocalization.

Useful links:

Fiji download

2. Advanced fluorescence microscopy

 FRET and detection of interactions in microscopy. Fluorescence lifetime and FLIM. Diffraction limit. Superresulution, STORM. PALM. Application to image actin fibrils.

M_L2-001.pdf (886.93 KB)
3. Fluorophores.

Fluorescent proteins. Small molecule dyes. Channel crosstalk and selection of fluorophores. Membrane trackers, staining of nuclei. Photodegradation during measurements. FRAP. Light intensity and damage to cells. Caged molecules and controllable photorelease. Photoswithchable molecules.

4. Atomic Force Microscopy. Electron microscopy.

Principle and scheme of microscopes. XY and Z resolution. Sample preparation. Scanning speed and sample damage. Application for protein unfolding. Principle. Resolution. Modes. Sample preparation.

Додаткові матеріали
Робота в ImageJ / Fiji

Завантаження безкоштовної програми Fiji  для роботи з мікроскопічними зображеннями.

Невеликий підручник по ImageJ/Fiji. 

Приклади файлів для самостійного освоєння програми:

Undergraduate students
4 Lectures
4 Weeks

Pathologist and Research Scientist with PhD.

Employee of the Precarpathian University (Ivano-Frankivsk) and the Institute of Organic and Bioorganic Chemistry (Prague). Doctorate degree in Life sciences (PhD) was obtained from Strasbourg University in 2009 for research work on the development of solvatochromic fluorescent labels for studies of protein interactions.

Senior research scientist Department of Neurophysiology, University of Tübingen

Предмети курсу

Course: 15 lectures, 6 seminars

Lectures: Halyna Semchyshyn, Galyna Ushakova, Oleh Lushchak

Seminars: Galyna Ushakova, Oleksandra Abrat

Course: 7 lectures, 4 seminars

Lectures: Volodymyr Shvadchak

Seminars: Volodymyr Shvadchak, Ivan Muzalov


Course: 9 lectures, 4 seminars

Lectures: Volodymyr Lushchak, Andriy Luzhetskyy

Seminars: Mariia Bailiak, Volodymyr Lushchak

Course: 13 lectures, 5 seminars

Lectures: Olga Garaschuk, Volodymyr Manko

Seminars: Volodymyr Manko, Volodymyr Merlavsky

Course: 4 lectures, 4 practical works

Lectures/Seminars: Volodymyr Shvadchak